藍色熒光蛋白TagBFP

藍色熒光蛋白TagBFP

藍色熒光蛋白TagBFP又名 mTagBFP，藍色熒光蛋白TagBFP- 亮藍色熒光 - 在融合中成功表現(xiàn)的單體蛋白 - 成熟快，光穩(wěn)定性高 - 極高的pH穩(wěn)定性，藍色熒光蛋白的激發(fā)光波長為402nm，發(fā)射光波長為457nm。

TagBFP是2008年發(fā)表的一種基本（組成型熒光）藍色熒光蛋白，來源于Entacmaea quadricolor。據(jù)報道，它是一種非常快速成熟的單體，具有非常低的酸敏感性。

TagBFP a.k.a. mTagBFP，TagBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 2008, derived from Entacmaea quadricolor. It is reported to be a very rapidly-maturing monomer with very low acid sensitivity.

藍色熒光蛋白TagBFP的光譜圖

藍色熒光蛋白用什么光激發(fā)？

從以上藍色熒光蛋白的激發(fā)和發(fā)射光譜圖可以看出，藍色熒光蛋白用紫光激發(fā)，發(fā)出藍色熒光。如果您用便攜式熒光蛋白激發(fā)光源，建議使用LUYOR-3280VI以及LUYOR-3415的V波段。

Blue fluorescent protein TagBFP

- Bright blue fluorescence

- Monomeric protein with successful performance in fusions

- Fast maturation, high photostability

- Extremely high pH-stability

- Recommended for protein labeling, acidic organelle labeling, FRET applications

TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.

Compared to EBFP2 [Ai et al., 2007], TagBFP is more then 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of TagBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.

Good overlap between the emission spectrum of TagBFP and the absorbance spectra of TagGFP2 allows using these two proteins as a FRET pair.

如果要觀察藍色熒光蛋白在斑馬魚上的表達，可以選用LUYOR-3421VI 臺式激發(fā)光源配合體視顯微鏡觀察。LUYOR-3421VI 臺式激發(fā)光源安裝有405-415nm的激發(fā)模組，能夠激發(fā)BFP熒光蛋白發(fā)出藍色熒光。